AmScope MU USB3.0 - Calibration Menu Procedure

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MU Series (Windows)
User’s Manual
Opons Menu Funcons
Calibraon Menu
The “Calibraon” opons allows the user to input a rule for the soware to use to determine how much of a
unit of real distance is equivalent to how many pixels the camera sees, at a given magnicaon. These rules are
used to perform calculated measurements while using the camera and soware, giving much needed exibility
for measuring small features in any type of a microscope. Note that this process must be repeated for every
magnicaon (and ideally, every unit of measurement) that the user intends to measure at. Using a seng at
a dierent magnicaon than calibrated will cause massive discrepancies between actual distance and mea-
sured distance.
1. Begin by switching the magnicaon on your microscope to your desired calibraon seng, and placing a
unit of known measurement on the stage. The smaller the division, the more accurate the measurement func-
ons will be, down to 0.01mm (the smallest guaranteed accuracy level of the camera and soware).
2. Focus on the micrometer slide or ruler while looking at the live feed. Once focused and set, adjust the live
resoluon to the highest available seng, set the zoom to 100%, and the unit of measurement to pixel, in the
upper toolbar.
3. Proceed to the “Opons” menu, and hit “Calibraon.” The above “Calibrate” menu will appear, with a red
line overlayed on the image. Adjust the line to posion it on the 0 point of your ruler, and the rst unit of mea-
surement (such as 1mm if using a ruler, or 0.01mm if using a micrometer slide), so that the line spans between
the two points.