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MU Series (Windows)
User’s Manual
Opons Menu Funcons
Calibraon Menu
The “Calibraon” opons allows the user to input a rule for the soware to use to determine how much of a
unit of real distance is equivalent to how many pixels the camera sees, at a given magnicaon. These rules are
used to perform calculated measurements while using the camera and soware, giving much needed exibility
for measuring small features in any type of a microscope. Note that this process must be repeated for every
magnicaon (and ideally, every unit of measurement) that the user intends to measure at. Using a seng at
a dierent magnicaon than calibrated will cause massive discrepancies between actual distance and mea-
sured distance.
1. Begin by switching the magnicaon on your microscope to your desired calibraon seng, and placing a
unit of known measurement on the stage. The smaller the division, the more accurate the measurement func-
ons will be, down to 0.01mm (the smallest guaranteed accuracy level of the camera and soware).
2. Focus on the micrometer slide or ruler while looking at the live feed. Once focused and set, adjust the live
resoluon to the highest available seng, set the zoom to 100%, and the unit of measurement to pixel, in the
upper toolbar.
3. Proceed to the “Opons” menu, and hit “Calibraon.” The above “Calibrate” menu will appear, with a red
line overlayed on the image. Adjust the line to posion it on the 0 point of your ruler, and the rst unit of mea-
surement (such as 1mm if using a ruler, or 0.01mm if using a micrometer slide), so that the line spans between
the two points.
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