4-18 Performance Verification
• The factory repeatability and accuracy tests that follow are
performed with Costar
flat (#3590) and round bottom (#3797)
microplates with Durkee
yellow food coloring, using a 405 nm
filter. Different solutions and microplates, may vary repeatability
and accuracy.
Accuracy (specification +/-1%+/-.010) @ 405nm to 2.00abs
1) Turn the microplate around (A1 is now in the H12 position and repeat the
test procedure described in the Repeatability section.
2) Compare the Means from each well, using the readings in the two positions.
The readings should be within 1% of the OD., and 0.010 counts of each
other. For example, if A1 was initially 1.000, then the "turn-around" test
should show results between .980 OD and 1.020 OD
Repeatability (specification +/- 1%+/-.005abs) @ 405nm to 2.00abs
1) Read the microplate using a single wavelength, no blanking (or blank on
air), and an 8 x 12 matrix format. Repeat the read six times.
2) Calculate the Mean and Standard deviation for each well. The Standard
Deviation should be within specifications for repeatability.
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