Appendix B B-17
If only a single strip is to be read, both bytes should be set to the same value, i.e. 0x03,
0x03 indicates read strip 3 only. If the last strip is lower than the first strip, only the
first strip will be read.
A first strip argument of 0x00 will cause the entire plate to be read (as will 0x01,
0x0C). By default the instrument will be initialized to perform full plate reads.
When the read is performed, initiated by the S command, response data will be
returned for only those strips indicated. As in full-plate reads, ODs will be presented
row-major, with each row terminated by <CR><LF>. For example, if strips 1-3 are
selected, data will be returned as A1, A2, A3, <CR>, <LF>, B1, B2, etc. Dual
wavelength subtracts, blanking, or multiple wavelength reads will be performed if
specified, in the same fashion as full plate reads. If blanking is selected, the blanking
wells will need to be included in the range of strips to be read.
NOTE: Data returned for each set of strips read at an indicated wavelength will
start with <CR> followed by that wavelength’s well ODs, as defined in the
previous section. Each set of strips will be terminated with a <^Z>
character followed by a checksum. Checksum calculation starts with the
first byte after the data start code (<CR>), up through and including the
<^Z> data terminator code. The checksum is transmitted as a single hex
data byte.
An aborted rea
d is indicated by a <DLE>
response at a
ny time. Any data received,
including checksums, should be considered invalid and irrelevant in this case.
Since the command function described above is new, there is no reason to maintain
compatibility with obsolete products. Therefore, no assay name, report title, or
time/date stamp, blank or otherwise, will be returned with data collected using the
above function.
If a strip range of 1 to 12 is selected, by default or otherwise, and no other new
command functions are involved (see Multiple Wavelengths, pg 18), data will be
returned using the protocol, as described in the previous section, including assay
name, report title, time/date stamp, and NO checksum.
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