SYSMEX CyFlow Cube 8 - Calibration and Count Check Beads (Low, Medium, High); Measurements and Data Acquisition

7 Troubleshooting
CyFlow
®
Cube 8 | Operating Manual | May 2018 | Revision 032 41
7.4 Calibration and Count Check Beads (low, medium, high)
7.5 Measurements and data acquisition
7.6
Observation Possible solution
The results of the
Count Check Beads
(low, medium, high)
measurements are
not within ± 10%
range of the
lot-specific
concentration stated
on the bottle.
Shake the bottle vigorously (e.g. by vortexing) and
repeat the measurement.
Check the date of expiry of the Count Check Beads
(low, medium, high).
Perform a priming procedure.
Perform a cleaning procedure.
The results of the
Calibration Beads
measurements are
not in the
preselected regions
of the original
configuration script.
Check in software if you have loaded the correct
configurations for your measurement.
Shake the bottle vigorously (e.g. by vortexing) and
repeat the measurement.
Check the date of expiry of the Calibration Beads.
Perform a priming procedure.
Perform a cleaning procedure.
Observation Possible solution
The device emits an
unusual / noisy
sound after starting
the measurement.
Check that the waste bottle is closed properly and
that the bottle is not cracked.
Check all visible tubes and make sure that they are
not pinched.
Check that liquid (sheath fluid and sample) is
dropping into the waste bottle after starting the
measurement.
Clean the two electrodes of the sample port
carefully by wiping them with a soft tissue soaked in
decontamination solution.
Perform a de-clog procedure.
There is no data
acquisition visible
(peaks/dots in
histogram/dot plot)
during the RUN
phase
Check in software if the laser/s is/are switched on.
Check if the right trigger parameter has been
selected (choose FSC trigger as default setting,
select SSC for very small cells, e.g. bacteria).
Check gain values and the threshold.
Increase the flow rate.
Perform a de-clog procedure.