6 Operation
30 CyFlow
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Cube 8 | Operating Manual | May 2018 | Revision 032
6.6 Software operation
The software can be run in two different modes:
In Worklist mode, data can be acquired. The hardware and software
settings for each measurement are predefined and organised in a list
called Worklist.
In Playlist mode, acquired data can be analysed and settings for future
measurements can be prepared. Files are organised and edited in a
Playlist.
This chapter describes how to use a Worklist for preparing sample
measurements and how to use a Playlist to analyse data. For a detailed
description of software functions, see chapter 12 Software description on
page 50.
6.6.1 Preparing measurements using the Worklist
This chapter describes the typical sequence of steps when preparing a
Worklist for future measurements.
A Worklist contains the predefinitions of hardware and software settings for
data acquisition (see chapter 12.6 Worklist pane on page 146). These
predefinitions are set prior to measurements when preparing the Worklist.
Preparing the Worklist includes:
Defining the Workspace that contains the plots, regions and gates to
represent the cell populations of interest;
Defining and adapting the instrument settings to reduce background and
to position the populations of interest properly into the plots;
Performing a manual compensation to compensate for fluorescence
spillover as appropriate.
These settings can be preset prior to acquiring data and adapted while
measuring samples.
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