SYSMEX CyFlow Cube 8 - Analysing Data and Creating Reports Using the Playlist

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6Operation

CyFlow

Cube 8 | Operating Manual | May 2018 | Revision 032 37

6.6.2 Analysing data and creating reports using the Playlist

Data can be analysed offline by using a Playlist (see chapter 12.7 Playlist

pane on page 154).

All data created for analyses, including plots, statistics, compensation

matrices and overlays, can be summarised in a page-based report that can

be printed.

Analysing data using the Playlist

Procedure

1. To create a new Playlist, select the File tab of the Ribbon (see chapter

12.4.1 File tab on page 58) and select "File > New > New Playlist".

In Worklist mode, a new Playlist can also be created by clicking the New

button in the Worklist pane (see chapter 12.6 Worklist pane on

page 146).

Alternatively, drag and drop a Playlist file from the Windows Explorer into

the Workspace or the Playlist pane.

– A new blank Playlist is opened and displayed in the Playlist pane (see

chapter 12.7 Playlist pane on page 154).

– The software is in Playlist mode.

2. To open a Playlist item in the Playlist, select the File tab of the Ribbon

and select "File > Open > Open Listmode".

Alternatively, click the Open Listmode button on the Quick Access

Toolbar, or click the Open Listmode button on the Home tab of the

Ribbon (see chapter 12.4.2 Home tab on page 65), or drag and drop an

FCS file from the Windows Explorer into the Playlist, or press Ctrl + O.

3. Plots, regions and gates can be edited in the Plots tab of the Workspace

as desired (see chapter 12.8.1 Plots tab of the Workspace on page 160).

4. Statistics can be displayed and edited in the Results tab of the

Workspace (see chapter 12.8.2 Results tab of the Workspace on

page 171).

5. In Playlist mode, for setting compensations, the Compensation Wizard

from the Compensation tab of the Ribbon can be used (see chapter

12.4.6 Compensation tab on page 84).

6. To save the compensation, select the File tab of the Ribbon and select

"File > Save > Save Compensation As".

7. For comparing plots to each other, overlay plots can be created manually

(see chapter 12.8.3 Overlays tab of the Workspace on page 174).

In Playlist mode, the Overlay Wizard on the Overlay tab of the Ribbon

can be used (see chapter 12.4.11 Overlay tab on page 109).

– The data are analysed.