Sparging Eluents 29
During pump operation, sparge all active reservoirs at a reduced rate (for example, 30
mL/min) with helium to:
• Maintain pump performance with blended eluents
• Prevent accumulation of dissolved air in the eluents
Note: A sparge rate of 50 mL/min is recommended for gradients involving methanol and
aqueous solutions.
If helium is not available or is undesirable for sparging, see Appendix B, Eluent
Considerations, for other degassing methods.
Preparing to Sparge
Adjust the helium tank pressure to a setting between 345 KPa and 1035 KPa (50 to 150
psi).
1. Confirm that sparge and eluent tubing are properly assembled and connected (see
the Waters 600E Multisolvent Delivery System Installation and Maintenance Guide,
Chapter 2, making Fluidic Connections to the 600E Pump).
2. Press the Setup function key. The Pump Setup screen appears (Figure 3-1).
Figure 3-1 Pump Setup Screen
Note: For eluents that contain minor volatile components, you can first sparge the eluents
without the component. Then, reduce the sparge rate and add the volatile component to
the sparged eluent.
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