Abbott CELL-DYN 3200 - Page 337

CELL-DYN
®
3200 System Operator’s Manual
6-39
9140181H—October 2001
Section 6 Calibration Procedures
9. To determine the New Calibration Factor for each parameter:
a. For Calibrator, use the values on the assay sheet and the CELL-DYN
mean values determined in steps 2 through 8 above. Enter this
information in columns 1 and 2 respectively of Worksheet 1, Open Mode
Calibration — New Factors, provided in Manual Calibration
Worksheets at the end of this section.
b. For Fresh Whole Blood, use the Reference Mean Values determined in
Determining Reference Values — Fresh Whole Blood described earlier
(refer to the Whole Blood Calibration Reference Values Worksheet) and
the CELL-DYN Mean values determined in steps 2 through 8 above.
Enter this information in columns 1 and 2 respectively of Worksheet 1,
Open Mode Calibration — New Factors, provided in Manual
Calibration Worksheets at the end of this section.
c. Enter the current Open Sampler Calibration Factors, obtained in step 3
above, in column 3 of Worksheet 1.
NOTE: Be sure to make copies of this worksheet as needed.
10. Follow the instructions on Worksheet 1 to calculate the new Open Mode
Calibration Factor for each parameter and enter this information in column 4
of the worksheet. The method for determining the new factors is:
a. Calibrator Calibration:
b. Whole Blood Calibration:
For example, if the Reference Mean Value for WOC is 6.6, the CELL-DYN
Mean for WOC is 7.1, and the current Open Mode Calibration Factor for
WOC is 0.98, then:
(6.6 / 7.1) × 0.98 = 0.91
and 0.91 is your New Open Mode Calibration Factor for WOC.
Determining Which Parameters Need Calibration
To determine which parameters require calibration in the Open Mode, follow the
procedure below:
1a. Transfer the values from column 4 of Worksheet 1 to the new Open Mode
Factor columns 1 and 3 of Worksheet 2, Open Mode Factor % Difference,
provided in Manual Calibration Worksheets at the end of this section.
NOTE: Make copies of this worksheet as needed.
1b. Transfer the values for the Current Open Mode Cal factor from column 3 of
Worksheet 1 and enter these values in column 2 of Worksheet 2.
2. Follow the instructions on this worksheet to determine the Factor % Diff for
each parameter.
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