6-40
CELL-DYN
®
3200 System Operator’s Manual
9140181H—October 2001
Calibration Procedures
Enter Factor Method
Section 6
3. Transfer the Factor % Diff values from column 5 (calculated in Worksheet 2)
to column 1 of Worksheet 3, Open Mode Calibration Range Criteria,
provided in Manual Calibration Worksheets at the end of this section.
NOTE: Make copies of this worksheet as needed.
4. For each parameter, if the Factor % Diff is equal to or less than the value in
the Lower Limit column, then CALIBRATION IS NOT REQUIRED for that
parameter because the value is within range.
5. For each parameter, if the Factor % Diff falls between the upper and lower
calibration range, shown in the Calibration Range column, then
CALIBRATION IS REQUIRED.
6. For each parameter, if the Factor % Diff is greater than the value in the
Upper Limit column, there may be a computation error or an instrument
problem. In this case, do the following:
a. Recheck all numbers and calculations on Worksheets 1 and 2.
b. Determine if any component on the instrument was changed. This could
affect calibration. Such components include the Shear Valve, Optical
Flow Cell, Hemoglobin Flow Cell, or one of the syringes.
c. If a component was changed, then treat the result as if it fell within the
“calibration range” (even though it is greater than the upper limit).
CALIBRATION IS REQUIRED for that parameter.
d. If no component was changed and your calculations are correct, DO
NOT CALIBRATE. Confirm that all Pre-Calibration procedures were
completed and call the Abbott Hematology Customer Support Center for
assistance (at 1-800-CELL-DYN in the U.S.).
7. Based on the results from steps 1 through 6 above, do one of the following:
a. If any parameter needs to be calibrated in the Open Mode, go to Entering
New Calibration Factors
—
Open Mode.
b. If none of the parameters requires Open Mode calibration and Closed
Mode has not been calibrated, go to Calibration Procedure — Mode to
Mode to determine if Closed Mode needs calibrating.
c. If none of the parameters requires Open Mode calibration and Closed
Mode calibration has already been completed, press [MAIN] to return to
the MAIN MENU.
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