3-50 Operation
If OD is chosen, the formula definition screen will appear. Use the formula keys (Math,
Other, Map and Function) to define the transformation variable (TVar). Once the
variable has been defined, it can be used in a transformation formula. The TVar will be
available as a MAP option when writing the transformation formula.
EXAMPLE 3:
An assay map has 2 blanks, 1 control well in duplicate (CTL1), 1 negative
control well in triplicate (NC), 5 standards in duplicate (STD1-STD5) in
varying concentrations.
The assay data reduction states:
• Subtract the mean of the NC minus the mean of CTL1, from all OD’s on
the plate.
• Divide by the mean of the NC minus the mean of CTL1, then multiply by
100.
On paper, the formula reads:
(OD - (NC;x - CTL1;x)) / (NC;x - CTL1;x) * 100
On the reader, the formula (NC;x - CTL1;x) will be programed as the
Transformation Variable, since the transformation will apply to all standards,
controls and samples on the plate. To do this:
• At the SCOPE VARIABLE selection, choose OD and press Enter.
• Now enter the formula (NC;x - CTL1;x) by using the Math, Other, Map and
Function keys. Press Enter.
• The regular formula definition screen is displayed. Choose TRANS.
• Enter the formula (OD - (Tvar)) / (Tvar) * 100, using the Math, Other, Map
and Function keys. (TVar is included in Map options on the formula entry
screen.) The transformation formula has been added to the assay definition.
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