ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
5.17
NON-GYNECOLOGIC SAMPLE PREPARATION
BODY FLUIDS
Body Fluids may include serous effusions, urinary and cerebrospinal fluids.
1. Collection: Collect body fluids fresh.
Note
: Fluids collected in CytoLyt
™
Solution also require a CytoLyt
Solution wash prior to instrument processing.
Note
: For extremely bloody fluids (i.e., pericardial), start with only
10 mL of fresh fluid.
2. Concentrate by centrifugation — 600g for 10 minutes (page 5.5) or
1200 g for 5 minutes.
3. Pour off supernatant and vortex to resuspend cell pellet (page 5.7)
4. CytoLyt Solution wash (page 5.12)
5. Evaluate cell pellet appearance (page 5.9)
If cell pellet is not free of blood, add 30 mL of CytoLyt Solution to cell
pellet and repeat from step 2.
6. Add an appropriate amount of specimen (dependent on the size of
the cell pellet) to PreservCyt
™
Solution vial (page 5.10)
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