12 Software description
170 CyFlow
®
Cube 8 | Operating Manual | May 2018 | Revision 032
The three phases of the DNA cell cycle are coloured on the cell cycle plot as
follows:
Red: Denotes the G0/G1 phase,
Blue: Denotes the S phase,
Green: Denotes the G2/M phase.
Additional regions cannot be applied to the cell cycle plot.
The following markers are displayed on the plot:
Blue Markers are debris markers allowing to set noise thresholds and to
exclude cellular debris. Events that occur below or above these noise
markers will be presented on the plot, but not analysed by the software.
The Red Marker marks the estimated mean channel of the G0/G1 peak.
The Green Marker marks the estimated mean channel of the G2/M
peak.
If the calculated phases are not as expected, the G0/G1 and/or G2/M
estimates can be adjusted by clicking on the marker and dragging/dropping it
to the desired position to generate a successful fit for the DNA cycle. The
estimate markers cannot be crossed over. The markers can only be moved
to within 10 channels (based on a 1024 channel histogram) from each other.
Noise markers cannot be moved outside the limits of the histogram.
Moving any of the markers triggers recalculation on the DNA cell cycle plot.
When the noise markers are moved, the automatically estimated G1/G0 and
G2/M peak values will be used. When the G0/G1 and/or G2/M are moved,
the adjusted values of these markers will be used.
Following a manual adjustment of the estimates, you can return the
calculated estimates to their original calculated positions. Click the Reset
Cell Cycle button on the Plot Format tab of the Ribbon to reset the cell cycle
with the automatically calculated G0/G1 and G2/M position estimates.
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