12 Software description
CyFlow
®
Cube 8 | Operating Manual | May 2018 | Revision 032 169
Plots added to the Plots area will display their scales on the X and Y axes.
Fluorescence parameters default to Log scaling. Scatter or other parameters
default to Lin scaling.
The scaling options are:
Linear Scales: Linear scaled parameters display linear tick marks and
numbering. The default values are determined from information in the
LMD file. Linear scaled data always starts at 0. The minimum and
maximum values are always displayed at the ends of the scale.
Log Scales: Logarithmic scaled parameters display logarithmic tick
marks and numbering. The default values are determined from
information in the LMD file. When no scaling information is available in
the opened LMD file nor in the Workspace, the scaling is determined by
the values found in the Scaling page of the software options (see chapter
Scaling page on page 189).
V-Log Scales: V-Log scaled parameters display a combination of linear
and logarithmic sections. Linear tick marks are displayed around the
origin and logarithmic tick marks are displayed towards the end of a
scale. When V-Log is selected, the full range of data is displayed.
To toggle between zoomed and unzoomed displays:
If one or more of the parameters associated with the plot have
non-default zoom settings, a Plot Zoom Toggle button is displayed on
the top left corner of the plot. Click the button to toggle between the
zoomed and unzoomed view of the plot.
Alternatively, use the Display zoomed button on the Parameters tab of
the Ribbon.
DNA cell cycle plots
You can perform DNA cell cycle analysis of single parameter linear data
plots. To display DNA cell cycle information, first select one or more
histograms and then press the Cell Cycle button on the Plot Format tab of
the Ribbon (see chapter Plot Type group on page 124).
If there are regions on any of the selected histograms, cell cycle cannot be
activated and the Cell Cycle button is disabled. You have to delete the
regions or deselect the plots containing them before cell cycle can be
activated. The DNA cell cycle plots are saved as part of the Workspace.
Each cell cycle plot is identified numerically with the title "Cell Cycle n",
where "n" is a number.
When hovering the cursor over a cell cycle plot, a tooltip is displayed listing
the cell cycle number, statistics and the error message associated with the
plot.