SYSMEX CyFlow Cube 8 - Page 33

6Operation
CyFlow
®
Cube 8 | Operating Manual | May 2018 | Revision 032 33
Adapting instrument settings during data acquisition
Procedure
1. Select the Settings pane (see chapter 12.5.3 Settings pane on
page 139).
2. In the "Voltage" column, adapt the voltage until the desired cell
populations are displayed in the proper range in the plots and can be
clearly separated.
When measuring an unstained control, edit the values for all
fluorescence parameters until the auto-fluorescence signals display
within the first/between the first and second decade of log scale.
3. In the "Threshold" column, adapt the threshold values to reduce
background for each parameter triggered. Increase or decrease the
threshold settings if the background is still too high or if the cell
population of interest is cut off.
– The instrument settings are adapted.
– The plots will automatically refresh according to the new instrument
settings.
Creating a region
To select the events of interest, regions can be created on the plots.
Procedure
1. Select the Home tab of the Ribbon (see chapter 12.4.2 Home tab on
page 65).
2. To preselect creating an elliptical region, a rectangular region, a
polygonal region, a linear region or a quadrant region, click the
corresponding button on the Region group (see chapter 12.4.2 Home tab
on page 65).
– The button is highlighted in orange.
3. For elliptical, rectangular or linear regions (on single-parameter plots),
draw out the region on the plot.
For a polygonal region, click on a plot to determine the first point of the
region, then click to define additional points. Complete the polygon by
clicking on the start point or by right-clicking.
For a quadrant region, click on the plot at the point you wish the
quadrant’s intercept point to appear.
4. To assign a name to the region, select the region and double-click the
region name on the plot. Enter a region name in the edit field or select a
name from the drop-down list.
Alternatively, select the region, select the Region Format tab of the
Ribbon (see chapter 12.4.15 Region Format tab on page 128) and use