6 Operation
34 CyFlow
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Cube 8 | Operating Manual | May 2018 | Revision 032
the "Region Name" box to enter a name or to select a name from the
drop-down list.
– A region is created.
Setting a parent gate
A region that contains all events of interest can be applied as a parent gate
to the other plots so that only the relevant events are displayed on these
plots. This gating will allow to focus on a subpopulation of cells only.
Procedure
1. In the Plots area of the Workspace, select the desired plot.
To select several plots, hold the Ctrl key while selecting or draw a
selection rectangle.
2. On the Home tab of the Ribbon (see chapter 12.4.2 Home tab on
page 65) click the arrow on the "Gate" box to expand a drop-down list
and select the desired region to be applied as a parent gate.
– A parent gate will be automatically created for the selected region.
– The parent gate will be applied to the selected plots, indicated by a
gate-coloured frame around the plots.
Stopping the measurement
If all described settings on the sample are set up, the measurement can be
stopped. When the sample is empty (the stop electrode is reached) or if a
stop condition is defined, measurement will stop automatically.
Procedure
1. On the Acquire pane (see chapter 12.5.4 Acquire pane on page 140)
click the Stop button.
– The measurement is stopped.
After finishing a measurement a dialogue box will appear. Press OK button
to proceed with an intermediate cleaning step. Please note that the
remaining sample will be aspirated during the cleaning step. Alternatively,
remove sample tube and press OK button. The cleaning step will be
performed with air. To skip the intermediate cleaning step press Cancel
button. The device is ready for the next sample measurement.
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