6Operation
CyFlow
®
Cube 8 | Operating Manual | May 2018 | Revision 032 35
Performing a manual compensation
A manual compensation can be performed online (during the measurement)
or offline (after data acquisition).
Procedure
1. On the Compensation tab of the Ribbon (see chapter 12.4.6
Compensation tab on page 84) click the Manual Compensation button
and the Plot Compensation button.
2. On the plot, draw the desired population of events into the required area
of the plot.
If using a quadrant, statistics are displayed on the Compensation tab of
the Ribbon and can be used to arrange the population. Negative events
are displayed in the lower left quadrant. A population negative for a
parameter on Y scale should be placed in the lower right quadrant to the
same height as the negative events. A population negative for a
parameter on X scale should be placed in the upper left quadrant to the
same range as the negative events.
For fine adjustment of compensation, use the arrow buttons on the
Compensation tab of the Ribbon. The direction of the arrow icons
indicates the way the plot populations move rather than the way the
compensation values change.
– A manual compensation is performed.
Saving the Workspace and the instrument settings
On completion of a sample measurement, an FCS file is automatically saved
containing the acquired data, the Workspace, the instrument settings and the
compensation. Workspace and instrument settings can additionally be saved
in separate files to use them independently.
Procedure
1. To save the Workspace of a Worklist item, select the File tab of the
Ribbon (see chapter 12.4.1 File tab on page 58) and select "File > Save
> Save Workspace" or "File > Save > Save Workspace As".
Alternatively, right-click the Worklist item in the Worklist to open the
context menu and select "Workspace > Save Workspace (As)".
– The Workspace is saved.
2. To save the instrument settings of a Worklist item, right-click the Worklist
item in the Worklist to open the context menu and select "Instrument
Settings > Save Instrument Settings".
– The instrument settings are saved.
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