•
Load chips in alphanumeric order (according to the chip ID printed on the lid) to
avoid data entry errors.
•
Apply the chip lid and add Immersion Fluid immediately after loading each chip
to avoid evaporation.
•
Load and seal chips in batches of up to 24 chips (the maximum number that can
be loaded onto one thermal cycler).
•
If you do not intend to load an opened chip immediately, cover the chip using the
plastic plate included in the chip package to prevent contamination. If you plan
to keep the opened chip for longer than a day, the chip should be stored with
dessicant.
•
Use all of the Immersion Fluid within 60 minutes of uncapping the syringe. After
a syringe is opened, you cannot recap it for later use.
•
Thermal cycle the chips within 2 hours after loading them.
Materials required
The following materials are required to load and seal chips for use on the
QuantStudio
™
3D Digital PCR System.
•
Prepared digital PCR reactions (from the previous section)
•
QuantStudio
™
3D Digital PCR 20K Chip v2
•
QuantStudio
™
3D Digital PCR Chip Lid v2
•
QuantStudio
™
3D Digital PCR Sample Loading Bladess
•
Immersion Fluid
•
Immersion Fluid tip
•
Isopropanol
•
Gloves, powder-free, nitrile
•
Heated block (capable of maintaining 40±1°C)
•
Scissors
•
Low-lint polyester wipes (clean-room grade)
•
Microcentrifuge
•
Pipettes and tips, P10 to P1000
•
Vortex mixer
Prepare the syringe containing the Immersion Fluid
1.
Remove the Immersion Fluid syringe, plunger, and tip from the packaging.
2.
Before uncapping the syringe, gently pull back the plunger 1-2 mm and release
it to break any resistance that may have formed during storage.
Appendix E
Load chips manually
Materials required
E
QuantStudio
™
3D Digital PCR System User Guide
107
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