Thermo Scientific Applied Biosystems QuantStudio - Operational Workflow

Operational workflow
The following shows a single experiment workow on the QuantStudio
™
3D
Digital PCR System. The procedures for sample and/or consumable preparation and
result analysis can vary depending on the specic experiment that you are
performing.
Start
q
Set up the dPCR reaction by mixing sample, master mix, and
assay(s).
q
Load the dPCR reaction onto a QuantStudio
™
3D Digital PCR
20K Chip v2, apply the lid, load the assembly with Immersion
Fluid, then seal the loading port.
q
Perform the PCR using the ProFlex
™
2 × Flat PCR System or Dual
Flat Block GeneAmp
™
PCR System 9700.
q
Read the chip using the QuantStudio
™
3D Digital PCR Instrument.
q
Review the results on the instrument touchscreen.
q
Store or discard the chip.
q
Analyze the data using the QuantStudio
™
3D AnalysisSuite
™
Software.
q
Finish
Note: See the user documentation for the QuantStudio
™
3D AnalysisSuite
™
Software
for detailed information on data analysis.
Chapter 2 Getting Started
Operational workflow
2
16
QuantStudio
™
3D Digital PCR System User Guide