Contents
■
CHAPTER 1 Product information ....................................... 8
About the QuantStudio
™
3D Digital PCR System .................................... 8
Instruments, kits, consumables, and accessories ................................... 9
Required materials not supplied ................................................. 10
Power line regulator ........................................................ 12
Surge protector ............................................................ 12
About the instrument ........................................................... 12
Data collection ............................................................ 13
Supported dyes ........................................................... 13
About the software ............................................................. 13
System requirements ....................................................... 13
About the chip ................................................................. 14
■
CHAPTER 2 Getting Started ........................................... 15
Operational workow ........................................................... 16
Connect the QuantStudio
™
3D Digital PCR Instrument to a network .................. 17
Connect the QuantStudio
™
3D AnalysisSuite
™
Software ............................ 17
Choosing a thermal cycler ....................................................... 17
Compatible thermal cyclers ................................................. 17
■
CHAPTER 3 Prepare the digital PCR reaction and load
the chips .................................................................. 19
Guidelines for loading and sealing chips .......................................... 19
Prepare the DNA samples ....................................................... 20
Quality of DNA ............................................................ 20
Quantity of DNA ........................................................... 20
Sample dilution ............................................................ 21
QuantStudio
™
3D Digital PCR System User Guide
3