Thermo Scientific Applied Biosystems QuantStudio - Page 4

Determine the optimal dilution when the target is known ........................ 21
Determine the optimal dilution when the target is unknown ...................... 22
Prepare the digital PCR reactions ................................................ 22
Required materials ......................................................... 22
Guidelines for dPCR sample preparation ..................................... 22
Recommended DNA volume and concentration ............................... 23
Prepare the dPCR reaction mix for each sample ............................... 23
Load the chips using the QuantStudio
™
3D Digital PCR Chip Loader ................. 24
Materials required ......................................................... 24
Chip loader status light ..................................................... 25
Prepare the chip loading workspace ......................................... 25
Prepare the syringe containing the Immersion Fluid ............................ 26
Load and seal the chips .................................................... 27
Fill the chip case with Immersion Fluid ....................................... 30
Seal the chip case with the label ............................................. 31
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CHAPTER 4 Thermal cycle the chips ................................. 32
Materials required .............................................................. 32
Prepare the thermal cycler ...................................................... 32
Guidelines for handling loaded chips ............................................. 35
Perform the PCR ............................................................... 36
Unload the thermal cycler ....................................................... 38
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CHAPTER 5 Image and analyze the chips ........................... 40
About imaging and primary analysis .............................................. 40
QuantStudio
™
3D System data ow .......................................... 40
About the instrument interface ................................................... 41
Chip imaging .................................................................. 42
Materials required ......................................................... 42
Guidelines for imaging multiple chips ......................................... 42
Specify the data destination ................................................. 42
Image the chips ........................................................... 43
Primary analysis ............................................................... 46
About imaging data and primary analysis ..................................... 46
About the results screen .................................................... 47
Data quality ags .......................................................... 48
About data les ............................................................ 48
Transfer the results ......................................................... 49
Using the QuantStudio
™
3D AnalysisSuite
™
Software .............................. 51
Access the software from a Connect cloud-based platform account ............. 51
Access the software from the server ......................................... 51
Obtain information from the Help system ..................................... 53
Contents
4
QuantStudio
™
3D Digital PCR System User Guide