Thermo Scientific Applied Biosystems QuantStudio - Data Collection; Supported Dyes; About the Software; System Requirements

The QuantStudio
™
3D Digital PCR Instrument collects raw uorescence data from the
QuantStudio
™
3D Digital PCR 20K Chip v2 following PCR amplication.
The instrument performs multiple image captures of the chip in the following three
phases:
1.
Excitation – The instrument illuminates all wells of the chip, exciting the
uorophores in each reaction.
2.
Emission Collection – The instrument optics collect the residual uorescence
emitted from the wells. The resulting image consists of light corresponding to
the range of emission wavelengths for the lter(s) in use.
3.
Interpretation
– The instrument assembles a digital representation of the
residual uorescence collected over a xed time interval.
After a run, the instrument determines the location and intensity of the uorescent
signals in each image, the dye associated with each uorescent signal, and the
signicance of the signal.
The QuantStudio
™
3D Digital PCR System features a lter set that is optimized for the
Applied Biosystems
™
FAM
™
, ROX
™
, and VIC
™
dyes.
About the software
The QuantStudio
™
3D Digital PCR System includes a software suite used to analyze
and manage digital PCR data generated by the QuantStudio
™
3D Digital PCR
Instrument. The QuantStudio
™
3D AnalysisSuite
™
Software includes a server and
Connect cloud-based platform deployment options that can satisfy a broad range of
laboratory requirements.
The QuantStudio
™
3D AnalysisSuite
™
Software is a web-based application that is
recommended for use with the following Internet browsers.
•
Google
™
Chrome
™
v37 or later
•
Microsoft
™
Internet Explorer
™
v10 or later
•
Mozilla
™
Firefox
™
v32 or later
•
Apple
™
Safari
™
v6 or later
Note: The software performance may vary based on your system conguration, and
requires an internet connection capable of 300kbps/300kbps (upload/download) or
better. If your network employs a rewall that restricts outbound trafc, it must be
congured to allow outbound access to apps.lifetechnologies.com on HTTPS-443.
Data collection
Supported
dyes
System
requirements
Chapter 1 Product information
About the software
1
QuantStudio
™
3D Digital PCR System User Guide
13