Plan the laboratory layout
When planning the placement of the QuantStudio
™
3D Digital PCR System
components, follow good laboratory practices for PCR. When preparing samples for
dPCR amplication, maintain separate areas and dedicated equipment and supplies
for pre-PCR (sample preparation, reaction setup, chip loading and sealing) and
PCR/post-PCR (amplication and chip reading). Do not bring amplied products into
the reaction setup area.
Room Activities Equipment
dPCR Setup
Work Area (pre-
dPCR room)
•
Sample preparation and DNA
extraction
•
Extracted DNA sample storage
•
dPCR reaction preparation and
DNA sample additions
•
Chip loading and sealing
•
General laboratory supplies (such as gloves and
wipes)
•
Microcentrifuge
•
Vortexer
•
Pipettes, P10 to P1000 with tips
•
Chips, chip lids, and loading blades
•
QuantStudio
™
3D Digital PCR Chip Loader (if used)
•
Immersion Fluid
Amplied DNA
Work Area
(PCR/post-PCR
room)
•
PCR amplication
•
Chip reading and storage
•
ProFlex
™
2 × Flat PCR System (or Dual Flat Block
GeneAmp
™
PCR System 9700)
•
QuantStudio
™
3D Digital PCR Instrument
Choose the instrument locations
Instrument location can affect performance. To prevent vibration, place instruments
on a solid, stable, level surface that allows free airow overhead and around the sides
and backs. To ensure optimal heat dissipation, keep all instrument ventilation slots
free of obstructions. For specic environmental requirements, see the following
references.
Instrument
For environmental requirements, see…
QuantStudio
™
3D Digital PCR
Instrument
“Environmental requirements” on page 82
ProFlex
™
2 × Flat PCR System ProFlex
™
PCR System User Guide
(Pub. No. MAN0007697)
Dual Flat Block GeneAmp
™
PCR
System 9700
GeneAmp
™
PCR System 9700 Base Module User
Manual (Pub. No. 4303481)
Appendix A Install the QuantStudio
™
3D Digital PCR System
Plan the laboratory layout
A
62
QuantStudio
™
3D Digital PCR System User Guide
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