Thermo Scientific Applied Biosystems QuantStudio - CHAPTER 4 Thermal Cycle the Chips; Materials Required; Prepare the Thermal Cycler

Thermal cycle the chips
■
Materials required .................................................... 32
■
Prepare the thermal cycler ............................................ 32
■
Guidelines for handling loaded chips ................................... 35
■
Perform the PCR ..................................................... 36
■
Unload the thermal cycler ............................................. 38
Materials required
•
Loaded and sealed QuantStudio
™
3D Digital PCR 20K Chip v2
•
ProFlex
™
2 × Flat PCR System (or Dual Flat Block GeneAmp
™
PCR System
9700)
•
QuantStudio
™
3D Digital PCR Chip Adapters
•
QuantStudio
™
3D Digital PCR Thermal Pads
•
QuantStudio
™
3D Digital PCR Tilt Base
•
Isopropanol
•
Gloves, powder-free, nitrile
•
Low-lint polyester wipes, clean-room grade
Prepare the thermal cycler
Before running each batch of chips, perform the following to ensure efcient thermal
cycling:
•
Conrm that clean QuantStudio
™
3D Digital PCR Thermal Pads are available for
use.
IMPORTANT! If a Thermal Pad becomes dirty, clean the pad using a low-lint
wipe sprayed with isopropanol, then let it dry. Fluids, debris, and other
contaminants that adhere to the pad can transfer to the chips during thermal
cycling and uoresce during imaging.
IMPORTANT! Use only Thermal Pads that are approved for use with chips.
Thermal compression pads from other PCR applications may not apply uniform
pressure or heat distribution to all chips on the sample block.
4
32
QuantStudio
™
3D Digital PCR System User Guide