•
Conrm that a QuantStudio
™
3D Digital PCR Tilt Base is installed beneath the
thermal cycler.
IMPORTANT! The Tilt Base inclines the sample block by 11°, the angle required
for the optimal performance of the chips.
•
Open the heated cover and conrm that QuantStudio
™
3D Digital PCR Chip
Adapters are installed to both sample blocks.
Note: Each Chip Adapter includes a set of four alignment pegs that t into the
holes of a at sample block. The Chip Adapter can t onto either side of the
sample block (right or left).
•
Conrm the thermal cycler is running the correct rmware version number to
thermal cycle chips:
–
ProFlex
™
2 × Flat PCR System—The version number displayed in the About
Instrument screen must be 1.1.4 or later.
About Instrument
Wired IP Address
Wireless IP Address
Instrument Serial Number
Block Serial Number
Firmware Version
10.0.2.15
12345678
12345678
1.1.4
b1765c31bd08370ba632d35ea56c505b
UUID
1
1
v1.1.4 or later
Note: If the ProFlex
™
2 × Flat PCR System version is earlier than 1.1.4, see
the ProFlex
™
PCR System User Guide (Pub. No. MAN0007697) for
information on upgrading the instrument rmware before thermal cycling
chips.
Chapter 4
Thermal cycle the chips
Prepare the thermal cycler
4
QuantStudio
™
3D Digital PCR System User Guide
33