Perform the PCR
CAUTION! PHYSICAL INJURY HAZARD. During operation, the sample block can
be heated to 100°C. Before performing the following procedure, be sure to wait until
the sample block reaches room temperature.
IMPORTANT! Thermal cycle prepared chips within 2 hours after loading them.
IMPORTANT! Thermal cycling must be performed on a ProFlex
™
2 × Flat PCR
System or a Dual Flat Block GeneAmp
™
PCR System 9700 as described in this
guide. No other thermal cyclers can be substituted at the time of the release of this
manual.
1.
Open the heated cover of the thermal cycler and wipe the surface of both
sample blocks using a low-lint wipe to ensure that they are clean and dry.
2.
Conrm that the Tilt Base is installed and Chip Adapters are installed in both
sample blocks (even if you are using only one block).
3.
Place the chips onto the sample block so that the ll ports on the chips are
positioned toward the front of the thermal cycler. The ll port must be elevated
during thermal cycling to ensure that any bubbles oat to the top of the case.
X0300036
Front of instrument
CAUTION HOT
24x
IMPORTANT! If you are thermal cycling less than 24 chips, load according to
the following guidelines:
·
Load the right sample block rst, placing at least 1 chip on the right sample
block.
·
Balance the load between the left and right blocks so that the pressure
applied by the heated cover and thermal pads is uniform across all of the
loaded chips.
Chapter 4 Thermal cycle the chips
Perform the PCR
4
36
QuantStudio
™
3D Digital PCR System User Guide
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