Thermo Scientific Applied Biosystems QuantStudio - Install the Quantstudio 3 D Digital PCR Instrument

Install the QuantStudio
™
3D Digital PCR Instrument
1.
Plan the organization of your laboratory. Identify the locations of all stations in
the QuantStudio
™
3D Instrument workow, including:
•
Reagent and consumable storage
•
PCR reaction preparation
•
Chip loading and sealing
•
PCR amplication on the ProFlex
™
2 × Flat PCR System or Dual Flat Block
GeneAmp
™
PCR System 9700
•
Chip reading on the QuantStudio
™
3D Instrument
Note: See “Plan the laboratory layout” on page 62 for more information on
planning the instrument placement.
2.
Conrm that you have the following materials:
•
QuantStudio
™
3D Digital PCR Instrument (with power cord)
•
QuantStudio
™
3D Digital PCR Chip Adapters
•
QuantStudio
™
3D Digital PCR Thermal Pads
•
QuantStudio
™
3D Digital PCR 20K Chip v2 (12-pack)
•
Base Module for the ProFlex
™
PCR System or the GeneAmp
™
PCR System
9700
•
ProFlex
™
2 × Flat PCR System or GeneAmp
™
PCR System 9700 Dual Flat
Block
•
QuantStudio
™
3D Digital PCR Tilt Base (appropriate for your thermal cycler)
•
QuantStudio
™
3D Digital PCR Chip Loader
If you have not received any of the items listed above, contact customer support
(see “Customer and technical support” on page 123).
3.
If you plan to connect your instrument to a network, conrm that you have one
of the following:
•
(Wired network) Category-6 Ethernet cable
•
(Wireless network) QuantStudio
™
3D Extended Arm WiFi Adapter
(Cat. No. A28598)
Note: Connecting the instrument to a network is optional and can be done later.
4.
Conrm that all chemistry kits and accessories have been stored at the correct
temperature.
5.
Unpack the QuantStudio
™
3D Instrument:
a.
Open the instrument package and conrm that you have received all parts
listed on the shipping manifest. If you are missing parts, contact customer
support.
b.
Remove the instrument and set it on a clean level surface, then remove the
protective cover.
Appendix A
Install the QuantStudio
™
3D Digital PCR System
Install the QuantStudio
™
3D Digital PCR Instrument
A
QuantStudio
™
3D Digital PCR System User Guide
63