Guidelines for handling loaded chips
To ensure the proper function of your loaded chips, handle them according to the
following guidelines:
•
Always wear powder-free gloves.
IMPORTANT! Oils from your hands can contaminate the chips and interfere
with imaging.
•
Handle the loaded chips by gently gripping the sides of each chip case, being
careful to not apply pressure to the chip within it.
•
Do not touch the window of the chip lid. If you accidentally touch the window,
clean it using a laboratory wipe that has been sprayed with isopropanol, then dry
by wiping in one direction using a clean low-lint wipe.
Note: Debris or excess sample on the lid can interfere with thermal uniformity
during thermal cycling, or uoresce during imaging.
•
Thermal cycle chips within 2 hours after loading them.
Chapter 4 Thermal cycle the chips
Guidelines for handling loaded chips
4
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3D Digital PCR System User Guide
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