Thermo Scientific Applied Biosystems QuantStudio - Load the Chips Using the Quantstudio 3 D Digital PCR Chip Loader; Materials Required

5.
Mix well by gently pipetting up and down (or gently vortex on low-medium
speed).
6.
Transfer 34.8 µL of the dPCR reaction mix to the labeled reaction tube.
7.
Cap the reaction tube, briey centrifuge, then immediately proceed to load the
chips.
IMPORTANT! For optimal results, load the chips as soon as possible after setting
up the reactions. If you placed the reactions on ice, warm them to room temperature
before loading.
Load the chips using the QuantStudio
™
3D Digital PCR Chip
Loader
For instructions on loading chips manually, see Appendix E, “Load chips manually”.
The following procedure describes how to load the QuantStudio
™
3D Digital PCR
20K Chip v2 automatically using a QuantStudio
™
3D Digital PCR Chip Loader. This
procedure assumes that you have prepared your PCR reactions as explained in
“Prepare the dPCR reaction mix for each sample” on page 23. Before proceeding,
see “Guidelines for loading and sealing chips” on page 19.
The following materials are required to load and seal chips.
•
Prepared digital PCR reactions
•
QuantStudio
™
3D Digital PCR 20K Chip v2
•
QuantStudio
™
3D Digital PCR Chip Lid v2
•
QuantStudio
™
3D Digital PCR Sample Loading Blades
•
Immersion Fluid syringe
•
Immersion Fluid syringe tip
•
QuantStudio
™
3D Digital PCR Chip Loader
•
Isopropanol
•
Gloves, powder-free, nitrile
•
Scissors
•
Clean room-grade, low-lint polyester wipes
•
Microcentrifuge
•
Pipettes and tips, P10 to P1000
•
Vortexer
Materials
required
Chapter 3 Prepare the digital PCR reaction and load the chips
Load the chips using the QuantStudio
™
3D Digital PCR Chip Loader
3
24
QuantStudio
™
3D Digital PCR System User Guide