–
GeneAmp
™
PCR System 9700—The version number displayed in the main
menu must be 3.12 or later.
08:00 AM 4/25/13 25.0°C
GeneAmp® PCR System 9700
Name:tc001 User:<<pe>>
F1 F3 F4 F5F2
Run UserUtilEditCreate
Version: 3.12
1
1
v3.12 or later
Note: If the GeneAmp
™
PCR System 9700 version is earlier than 3.12,
contact technical support to upgrade the instrument rmware before thermal
cycling chips (see “Customer and technical support” on page 123).
•
Conrm that the thermal cycler is programmed with the method for thermal
cycling chips. If missing, create the PCR method before proceeding.
Note: See the ProFlex
™
PCR System User Guide (Pub. No. MAN0007697) or the
GeneAmp
™
PCR System 9700 Base Module User Manual (Pub. No. 4303481) for
more information on programming methods.
IMPORTANT! The heated cover MUST be at 70°C before loading the chips.
Excessive heat from the heated cover may cause the chips to leak during
thermal cycling.
Table 2 ProFlex
™
2 × Flat PCR System PCR Method
PCR protocol
Cover
temp.
Reaction
volume
Stage 1 Stage 2 Stage 3
96.0°C 60.0°C 98.0°C 60.0°C 10.0°C
70.0°C 1 nL
[1]
1.6°C/sec 1.6°C/sec 1.6°C/sec 1.6°C/sec 1.6°C/sec
0:10:00 0:02:00 0:00:30 0:02:00 ∞
1x (Hold) 39x (Cycles) 1x (Hold)
[1]
33 nL for firmware earlier than v1.1.4. The reaction volume on the instrument display does not refer to
the reaction volume on the chip and should not be changed.
Table 3 GeneAmp
™
PCR System 9700 PCR Method
PCR protocol
Run
speed
Reaction
volume
Stage 1 Stage 2 Stage 3
96.0°C 56.0°C 98.0°C 60.0°C 10.0°C
Standard 20 μL
[1]
10 min 2 min 30 sec 2 min ∞
1x (Hold) 39x (Cycles) 1x (Hold)
[1]
The reaction volume on the instrument display does not refer to the reaction volume on the chip and
should not be changed.
Chapter 4
Thermal cycle the chips
Prepare the thermal cycler
4
34
QuantStudio
™
3D Digital PCR System User Guide
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