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Waters 2475 - Page 102

Waters 2475
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3-30 Using the Detector
Instrument setup
To set up the instrument:
1. On the configuration screen (Shift, DIAG), configure the detector to
operate in 474 Emulation mode.
2. Connect the detector via a standard RS-232 cable to any available COM
port on a Millennium
32
PC.
3. In the operating system’s device manager, check to ensure the COM port
you connected the detector to is available.
Tip: The same parameters apply to a 474 detector.
Method parameters
The initial conditions for the method are specified on the General tab of the
fluorescence method editor in Empower or Millennium
32
software.
Tip: The 2475 detector and the 474 detector interpret some method
parameters differently.
PC configuration for remote control
Parameter Value
Baud rate 4,800
Stop bits 2
Parity None
Data length 8 bits
Flow control Xon/Xoff
Example of method parameters
Parameter Value
Excitation λ (nm) 350
Emission λ (nm) 397
Bandwidth (nm) 18
Filter Type Digital
Filter Response 10

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