3-38 Using the Detector
can also use the Energy setting on channel B while making the primary
fluorescence measurement specified by a EUFS on channel A. For
example, while operating in single-channel mode, you can set an EUFS
of 500 on the second channel, providing a different scaling factor on the
channel B 1-V output.
The detector engages the second-order filter for all excitation wavelengths
greater than 400 nm.
You can configure the detector to show the measurement results in either
emission units or energy units (see page 3-19).
Operating the detector in multichannel mode
You can operate the detector with expanded chart-out selections in
multichannel (λλ) mode, which offers the following functions:
• Emission (A and B)
• Sample energy (A and B)
• Reference energy (A and B)
•MaxPlot
• Difference (A-B) or (B-A)
For details about these functions, see page 3-19 and the table titled “Primary
and secondary function (method) parameters” on page 3-21.
Changing from single to multichannel mode
To change from single to multichannel mode:
1. From the home screen in single-channel mode (λ), select λ/λλ (Shift,
Auto Zero).
This key toggles between single and multichannel mode, and the
detector displays a message indicating it is setting up multichannel
operation.
2. Specify the excitation wavelength to monitor in the xλ field, and then
press Enter.
3. Specify the emission wavelength to monitor in the eλ field, and then
press Enter.
To Next Page
Loading...
