Waters 2475 - Operating the Trace and Scale Functions

Preparing to start a run 3-23
Operating the trace and scale functions
The trace function displays a fluorescence signal for the last n minutes (up to
and including 60) of detector operation.
• Press TRACE to display fluorescence acquired over the last 30 minutes
by default. The trace is updated once every 20 seconds.
• Select Scale (Shift, TRACE) to display the scaled trace, which shows T1,
the ending time (-30 for the last 30 minutes), by default.
You can change the ending time, specifying any number from 3 through
60. You can use the scale function to zoom in on a particular section of
the trace.
Displaying the scale parameters
To display the scale parameters:
1. Press Scale.
2. Press Next to display T2 (starting time). The default is 0.
3. Press Next again to display EU1 (starting or low fluorescence). The
default is auto.
4. Press Next again to display EU2 (ending or high fluorescence). The
default is auto.
By entering appropriate times and fluorescence numbers in the four
scaling parameter boxes, you can zoom in on one section of an ongoing
fluorescence trace.
• For EU1 and EU2, press CE to reset to auto.
Auto Zero on
inject
4 (of 4) Check
box
None Checked
not checked
Checked
Auto Zero on
λ changes
4 (of 4) Choice None • To
baseline
•To zero
• Disable
To baseline
Primary and secondary function (method) parameters (Continued)
Function Screen Type Units Range Default