Operating the detector 3-33
the data on the channel A analog output, not the data output to
Empower or Millennium
32
software through the RS-232 connection.
•Auto Zero – Corresponds directly with enabling (Auto) and disabling
(Manual) the automatic Auto Zero on injections, gain, or wavelength
changes. You change these settings via the boxes on page 4 of the 2475
operator interface. If you select manual, the detector performs an Auto
Zero only when directly commanded to do so, either as a timed event,
front panel button, or rear panel (terminal block) contact closure. If you
select auto, it performs an Auto Zero at the start of any injection or when
the wavelength or gain is changed.
•Polarity – Defines the polarity of the analog output only.
Operation details
• Inject Start – The detector is configured with a switch-closure signal
input to the chart mark terminal on the rear panel. A signal input starts
injection run timer. You can also attach the wire to the inject start
terminal on the rear panel.
• Compatibility with the Waters 474 detector – Separation methods for
the 2475 and 474 detectors are compatible except for their gain values.
See page 3-19, “Emission (A & B)” and “Sample Energy (A & B).” The
higher sensitivity of the 2475 detector’s flow cell produces significantly
higher signals. Thus gain values optimized for the 474 detector can yield
474 and 2475 attenuation values
474 attenuation constants 2475 translation to EUFS
S (short) 1
11
210
450
8 100
16 500
32 1,000
64 5,000
128 10,000
256 100,000
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