1-1
1 Theory of Operation
This chapter explains the theory and technology supporting the
operation of the Waters
®
2475 Multi λ Fluorescence Detector and
describes the instrument’s features.
Contents:
Topic Page
Fluorescence theory 1-2
Fluorescence detection 1-3
Measuring fluorescence 1-4
Detector description 1-7
Principles of operation 1-9
Operational modes 1-14
Spectrum scanning 1-16
Lamp energy and performance 1-16
Auto-optimize gain 1-17
Startup diagnostic tests 1-21
Mobile-phase solvent degassing 1-21
Wavelength selection 1-22