Waters 2475 - Page 213

Wavelength selection C-13
Aldehyde —CHO 210 strong 280–30
0
11–18
Carboxyl —COOH 200–
210
50–70
Sulfoxide >S→O 210 1500
Nitro —NO
2
210 strong
Nitrite —ONO 220–
230
1000–2000 300–40
0
10
Azo —N=N— 285–
400
3–25
Nitroso —N=O 302 100
Nitrate —ONO
2
270
(shou
lder)
12
Allene —(C=C)
2
—
(acyclic)
210–
230
21,000
Allene —(C=C)
3
— 260 35,000
Allene —(C=C)
4
— 300 52,000
Allene —(C=C)
5
— 330 118,000
Allene —(C=C)
2
—
(alicyclic)
230–
260
3000–8000
Ethylenic/
Acetylenic
C=C—C≡C 219 6,500
Ethylenic/Amido C=C—C=N 220 23,000
Ethylenic/
Carbonyl
C=C—C=O 210–
250
10,000–20,
000
Ethylenic/Nitro C=C—NO
2
229 9,500
Wavelength selection for chromophore detection (Continued)
Chromophore
Chemical
Configuration
λ
max
(nm)
∈
max
(L/m/cm)
λ
max
(nm)
∈
max
(L/m/cm)