Priming the Pump 33
Drawing Off Eluent
Once you have initiated flow, draw off eluent as follows:
1. Move the cursor to the %A field for the first eluent and type 100 in the New column.
Press Enter.
2. Type 0 and press Enter for the remaining eluent composition fields.
3. Attach the priming syringe to the Luer™ fitting on the inlet manifold valve
(Figure 3-4).
4. Rotate the inlet manifold valve handle fully counterclockwise to the Draw position
(see Figure 3-3). Draw off 10 mL of eluent, rotate the handle to the Run position,
remove the syringe and discard the eluent properly.
Figure 3-4 Drawing Off Eluent
5. Repeat steps 1 through 4 for each of the remaining reservoirs that you intend to use.
Priming
1. Attach the priming syringe to the Luer fitting on the inlet manifold valve (see
Figure 3-4).
2. Move the cursor to the %A field for the first eluent and type 100. Press Enter. Type
0 and press Enter for the remaining % composition fields.
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