100 Agilent 1200 Infinity Series DAD User Manual
5 Optimizing the Detector
Optimizing the Detector Regarding to the System
Optimizing the Detector Regarding to the System
Delay Volume and Extra-Column Volume
The delay volume is defined as the system volume between the point of mixing
in the pump and the top of the column.
The extra-column volume is defined as the volume between the injection point
and the detection point, excluding the volume in the column.
Extra-Column Volume
Extra-column volume is a source of peak dispersion that will reduce the
resolution of the separation and so should be minimized. Smaller diameter
columns require proportionally smaller extra-column volumes to keep peak
dispersion at a minimum.
In a liquid chromatograph the extra-column volume will depend on the
connection tubing between the autosampler, column and detector; and on the
volume of the flow cell in the detector. The extra-column volume is minimized
with the Agilent 1290 Infinity/Agilent 1260 Infinity LC system due to the
narrow-bore (0.12 mm i.d.) tubing, the low-volume heat exchangers in the
column compartment and the Max-Light cartridge cell in the detector.
How to Configure the Optimum Delay Volume
To maintain resolution in the Agilent 1290 Infinity/Agilent 1260 Infinity
Diode-array Detector the 10 mm Max-Light cartridge cell has a low dispersion
volume (σ volume 1.0 μL) and no further volume optimization is required. In
situations where the alternative 60 mm Max-Light high sensitivity cell is used
to get higher sensitivity the cell volume is optimized for the use with 3 mm and
4.6 mm inner diameter columns.
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