154 Agilent 1200 Infinity Series DAD User Manual
8 Test Functions and Calibration
Wavelength Verification Test
Wavelength Verification Test
The detector uses the alpha (656.1 nm) and beta (486 nm) emission lines of
the UV-lamp for wavelength calibration. The sharp emission lines enable
accurate calibration. When verification is started, the 1-nm slit is moved into
the light path automatically. The test is run with the Max-Light Cartridge Cell
or with Max-Light Cartridge Test Cell installed.
If the test is performed with the Max-Light Cartridge Test Cell, the test results
are not influenced by solvent or pump effects.
When The detector is calibrated at the factory, and under normal operating conditions should not require
recalibration. However, it is advisable to recalibrate:
• after repair of components in the optical unit,
• after exchange of the optical unit or main board,
• after replacing the Max-Light Cartridge Cell or UV-lamp,
• after significant environmental condition changes (temperature, humidity),
• at a regular interval, at least once per year (for example, prior to an Operational
Qualification/Performance Verification procedure), and
• when chromatographic results indicate the detector may require recalibration.
Parts required # Description
1 Max-Light Cartridge Test Cell or
1 Max-Light Cartridge Cell
Preparations • Lamp must be on for at least 10 minutes.
• When using a Max-Light Cartridge Cell a flow rate of 0.5 mL/min with water is required.
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