82 Agilent 1200 Infinity Series DAD User Manual
5 Optimizing the Detector
Optimization Overview
Inline Pressure Relief Valve Kit (G4212-68001)
When several detectors are installed in a system the connecting capillary and
fittings between the detectors must be carefully chosen to keep
chromatographic influence on peak shape small. On the other hand narrow
bore connection capillaries generate a significant pressure drop dependent on
flow rate and solvent properties.
The pressure relief valve is designed to protect the flow cell of a Agilent 1200
Series Infinity Diode Array Detector (G4212A DAD and G4212B DAD ). Agilent
strongly recommends installing the pressure relief valve at the outlet of the
detector as soon as a second detector is installed like in LC/MS applications.
The pressure relief valve with a low internal volume check valve. The dead
volume is smaller than 100 nL delay volume (inlet to outlet). The ball of the
check valve is spring loaded and adjusted to open at typically 100 bar. On
overpressure (typically around 100 bar) it releases the pressure to waste.
Application Information
For the analysis and characterization of proteins and large biomolecules for
SEC, AEX and RP applications add 100 mM salt into mobile phase or 10 %
organic to prevent secondary interaction.
For cation exchange chromatography the usage of an Agilent Diode Array
Detector G1315C/D with the respective bio-inert flow cell is highly
recommended to avoid unspecific interaction of the protein with the flow cell.
For applications with mobile phases of a pH above 12.5 use an Agilent Diode
Array Detector G1315C/D and the respective bio-inert flow cell.
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