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84 Agilent 1200 Infinity Series DAD User Manual

5 Optimizing the Detector

Optimization Overview

Special Information for Bio-Inert Cartridge Flow Cells

For bio-inert applications use the specified BIO Max-Light Cartridge Flow Cell

only.

Both bio-inert Max-Light Cartridge Flow Cells include

• PEEK tubing 1/16” (0890-1763) and

• Fingertight fitting long (5062-8541)

Recommendations

Assure the following:

• The capillary ends are right-angled when cutting capillaries.

• No pliers or wrenches are used to fix the PEEK fittings at the flow cell.

• No metal ferrules are used at the cell unions to prevent contaminations and

damage.

• the flow cell is bypassed when flush procedures at pH > 12.5 are used.

Application Information

For the analysis and characterization of proteins and large biomolecules for

SEC, AEX and RP applications add 100 mM salt into mobile phase or 10 %

organic to prevent secondary interaction.

For cation exchange chromatography the usage of an Agilent 1260 Infinity

Diode Array Detector G1315C/D with the respective bio-inert flow cell is

highly recommended to avoid unspecific interaction of the protein with the

flow cell.

For applications with mobile phases of a pH above 12.5 use an Agilent 1260

Infinity Diode Array Detector G1315C/D and the respective bio-inert flow cell.

Agilent Technologies 1200 Infinity Series User Manual

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