Agilent 1200 Infinity Series DAD User Manual 83
Optimizing the Detector
5
Optimization Overview
Special Information of 60 mm Cartridge Flow Cell
Application Information
The geometrical volume of the 60 mm cell is 6 times larger than the 10 mm
cell. However, the chromatographic relevant dispersion volume, the square
roots of variances, accounting for cell specific geometrical volume shape and
fluidic flow pattern, have been determined as σV = 4 μL and σV = 1 μL in for
the 10 mm cell.
Due to the larger dispersion volume, the 60 mm cell is primarily designed for
4.6 mm column applications to achieve highest sensitivity with no additional
peak broadening. However, if sensitivity is important the 60 mm cell will also
be advantageous in case of smaller columns (3 mm, 2.1 mm) but depending on
the chromatographic system and method additional peak broadening might
occur.
The upper limit of concentration
Care should be taken in methods where high background absorption of
solvents or modifiers are present. When using the 60 mm cell the detector will
measure 6 times the background absorption as in case of the 10 mm cell,
which will reduce the remaining dynamic absorbance range for sample peaks.
Furthermore those UV absorbing modifiers could compromise the sensitivity
gain (signal/noise) of 60 mm cell.
The linearity limit of the detector is seen at about 2 AU for both, the 10 mm
and the 60 mm Max-Light Cartridge Flow Cell. Using firmware revision
B.06.25 and below, the 60 mm Max-Light Cartridge Cell linearity limit would
be 333 mAU/cm.
Required Detector Firmware
For use of the 60 mm Max-Light Cartridge Flow Cell a detector firmware
B.06.26 (introduced December 2009) or later is required.
If the 60 mm Max-Light Cartridge Flow Cell is used with detector firmware B.06.25 and
below, the detector output (digital and analog) is normalized to 1 cm. This means the peak
height would be the same as on the 10 mm Cartridge Flow Cell, the noise is reduced by a
factor of 6 and the linearity limit would be 333 mAU/cm.
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