58 Agilent 1200 Infinity Series DAD User Manual
4 Using the Module
Setting up the Detector with Agilent ChemStation
General Method Settings
Signals
Up to 8 individual signals can be set. For each of the signals,
the wavelength and bandwidth can be set for sample and
reference.
Limits:
Wavelength: 190.0 to 640.0 nm in steps of 0.1 nm
Bandwidth: 1.0 to 400.0 nm in steps of 0.1 nm
Setting an appropriate reference wavelength could improve
the baseline behavior. Alternatively use the 1.6 µL heat
exchanger from the G1316C TCC or an optional DAD heat
exchanger (if available).
Peakwidth
Peakwidth enables you to select the peak width (response
time) for your analysis. The peak width is defined as the
width of a peak, in minutes, at half the peak height. Set the
peak width to the narrowest expected peak in your
chromatogram. The peak width sets the optimum response
time for your detector. The peak detector ignores any peaks
that are considerably narrower, or wider, than the peak
width setting. The response time is the time between 10 %
and 90 % of the output signal in response to an input step
function. When the All spectrum storage option is selected,
then spectra are acquired continuously depending on the
setting of the peak width. The time specified by the peak
width is used as a factor in the acquisition of spectra. The
acquisition time for one spectrum is slightly less than the
peak width divided by 8, which is the acquisition time.
Limits: When you set the peak width (in minutes), the
corresponding response time is set automatically and the
appropriate data rate for signal and spectra acquisition is
selected.
• Do not use peak width shorter than necessary.
• G4212B: Do not use 0.025 seconds response time (no
filtering/high noise and no need (actually ultra-fast LC
doesn't deliver peaks < 0.0025 min/ < 0.15 sec).
The 1290 Infinity DAD (G4212A) has a data rate of up to 160 Hz.
The 1260 Infinity DAD (G4212B) has a data rate of up to 80 Hz.
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