86 Agilent 1200 Infinity Series DAD User Manual
5 Optimizing the Detector
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion
As a rule-of-thumb the flow cell volume should be about 1/3 of the peak
volume at half height. To determine the volume of your peaks, take the peak
width as reported in the integration results multiply it by the flow rate and
divide it by 3).
Traditionally LC analysis with UV detectors is based on comparing
measurements with internal or external standards. To check photometric
accuracy of the Agilent detector it is necessary to have more precise
information on path lengths of the detector flow cells.
This may result in problems when the used peak width is set to large and all peaks are
filtered accordingly.
Part Number Path Length
Cell Volume (σ)
G4212-60008/G5615-60018 1.0 cm 1.0 µL
G4212-60007/G5615-60017 6.0 cm 4.0 µL
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