178 BD High Throughput Sampler User’s Guide
controls, acquisition 30
conventions, user’s guide x
copy and paste
instrument settings 45
wells and specimens 42
coupler, sample
inspection and service 109
replacing 123
shown 17, 18
creating compensation controls 37
customer support xi
cytometer
connectors 19–21
interface panel 19–21
sample injection tube (SIT) 21
setting up 59
support bracket, shown 20
cytometer settings
optimizing 78–80
D
daily cleaning 104–108
daily maintenance 104–110
data
acquiring 82–83
analyzing 86–93
dead volume 25
decontaminating
external surfaces 167
fluidics
automatically 111
manually 163
defaults
acquisition settings 14, 37
HTS settings 40
deleting wells and settings 40
depot repair See repair procedures.
door sensor cable, BD FACSCanto II 18
E
error messages
acquisition 147
hardware 146
software 149
experiment
creating 64
exporting 84
templates 84
Experiment Layout 71–73
accessing from shortcut menu 40
events to record 72
shortcut keys 73
labels 71
exporting
experiment templates 84
panel template 85
plate template 76
well settings and files 40
external surfaces, decontaminating 167
F
fittings
hexagonal, inspecting 143
thumbscrew, inspecting 142
tightening 124, 133, 143, 160
fluidic modes
fluidics shutdown 101
fluidics startup 58
sheath exchange 99
fluidics
cleaning 163
manually decontaminating 163
modes 14
rinsing 165
shutdown, BD FACSCanto 100
startup, BD FACSCanto 58
tubing, shown 17, 18
fluorophore labels 37
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