Chapter 2: BD FACSDiva Software Overview 47
• Pause/Resume toggle button—pauses the sequence after finishing collection
of the current well; resumes a paused sequence with the next well in the
sequence
During an uninterrupted sequence, BD FACSDiva software calculates the
acquisition time of each well based on sample volume. This acquisition time is
referred to as the stopping time, and is calculated as follows:
The software will stop acquisition or recording of a well and proceed to the next
well when any of the following stopping rules have been met:
• the specified number of events were collected
• the stopping time was reached
• the file exceeded memory specifications
NOTICE If an HTS error occurs during a run, the run will abort and an error
message will display. Once the sequence run is complete, a dialog will be
displayed with the status of the run and whether any errors occurred. An alert
will sound until the dialog is dismissed.
Running Wells Manually
When running samples in manual mode, use the Basic Controls in the Acquisition
Dashboard. You can acquire, record, and restart acquisition on a single selected
well as long as a sequence is not currently running. For detailed information on
the Basic acquisition controls, refer to the BD FACSDiva Software Reference
Manual.
To start manual mode, do one of the following:
• Click on a well and then click Acquire Data under Basic Controls to start
acquisition
stop time (msec) =
sample volume (µL)
flow rate (µL/sec) x 1 sec/1000 msec
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