Biotek 405 LS - Page 100

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80 | Chapter 3: Operation

Advanced Settings

Do not change the slope or offset values unless specifically instructed to do so by

BioTek.

Note: Instructions in the Replacement Procedure for the Verify Test Plate may

recommend changing these values.

405™ Microplate Washer LS

Main Page

Notices4
Trademarks4
Restrictions and Liabilities4
Table of Contents5
Contact Information10
Customer Service and Sales10
Service/Tac10
European Coordination Center/Authorized European Representative10
Document Conventions11
Document Revision History11
Intended Use Statement11
Quality Control12
Warranty and Product Registration12
Repackaging and Shipping12
Warnings12
Hazards and Precautions13
Hazards13
Precautions14
CE Mark16
Directive 2004/108/EC: Electromagnetic Compatibility16
Directive 2006/95/EC Low Voltage (Safety)17
Directive 2002/96/EC: Waste Electrical and Electronic Equipment17
Directive 98/79/EC: in Vitro Diagnostics (if Labeled for this Use)17
Electromagnetic Interference and Susceptibility17
Usa Fcc Class a17
Canadian Department of Communications Class a XVI18
User Safety18
Safety Symbols19
Introduction21
Introducing the 405™ Microplate Washer LS22
Instrument Models23
Biostack Compatibility23
Package Contents25
Waste and Dispense System Accessories26
Optional Accessories27
Physical Specifications29
Performance Specifications30
Biotek's Customer Resource Center32
Installation33
Unpack and Inspect the Instrument34
Remove the Shipping Hardware34
Setting up the 405 LS38
Install the Microplate Carrier38
Connect the Vacuum Pump, Tubes, and Bottles38
Waste System39
Install the Vacuum Line Filter41
Install the Fluid Supply System42
Final Check44
Attach the Mist Shield44
Install Software/Connect to Computer45
Connect to Host Computer45
Connect to Power46
Define Instrument Settings46
LHC Users Only46
Standard Vacuum Pump Users47
Define Startup Preferences (LHC Users Only)47
Verify Performance49
Verify the Washer50
Run the Verify Test ("Q" Models Only)50
Repacking the 405 Washer52
Operation57
Basic Operation58
About the 405 LS Wash Step58
Optimize Performance59
405 Recommendations for Priming the Washer59
Optimize Protocols to Improve Evacuation60
Running Predefined Protocols61
Creating Protocols: Washing, Aspirating and Dispensing Fluid61
LHC Users Only: Customize the Predefined Protocols61
Predefined Protocols Listing63
Predefined Sample Protocols64
Introducing the 405 LS Keypad66
Quick Wash (Keypad Only)67
Quick Prime (Keypad Only)68
Create or Edit a Protocol (Keypad Only)68
How to Name a Protocol (Keypad Only)69
Define the Plate Type and Plate Map (or Partial Plate)70
How to Shake the Plate71
How to Enter Negative Numbers (Keypad Only)72
How to Copy a Protocol72
How to Delete a Protocol72
Using LHC to Control the 405™ Washer74
Introducing the LHC Workspace74
Communications Port75
405 Steps75
LHC Protocols76
LHC Protocols Explained77
Target Instrument Settings78
How to Define a Protocol (LHC Only)79
How to Use the Adjust Utility- LHC80
Upload-Download Protocols (LHC Only)82
Wash Step Parameters Table82
Shake/Soak Step Parameters86
Pre-Dispense87
Washer Flow Rates88
How to Define a Final Aspiration Step89
Add a Secondary Aspiration to a Wash Cycle90
Bottom Wash90
Verify Performance92
Handling the Verify Test Plate93
Verify™ Technology Flowchart Diagram95
Verify Test Corrective Action95
About Verify™ Technology96
Sensitivity: Verify Sensor Vs. Absorbance Reader98
Configure Verify™ Technology Utilities99
Operating with the Biostack101
Biostack Alignment Utility102
Cell Wash104
Cell Wash Strategies105
Biomagnetic Separation - Magnetic Bead Assays109
Perform Magnetic Bead Assays110
Optimize Magnetic Bead Protocols110
Flat Magnets111
Ring Magnets112
Magnet Height Offset113
Determine Magnet Height Offset113
Manifold Stop Screw Adjustment Kit114
Vacuum Filtration for Filter Plate Assays117
Install the Side Bracket118
Set up the Vacuum Filtration Module119
Enable Vacuum Filtration121
Create a Vacuum Filtration Protocol121
Install Vacuum Filtration Plate Carrier123
Control the Vacuum Level124
Washer Settings125
Change the Washer Manifold Setting (Keypad)127
Change the Plate Carrier Setting (Keypad)127
Change the Plate Clearance Setting (Keypad)128
Adjust Utility128
Run the Adjust Utility (Using the Keypad)129
Plate Types and Processing Patterns130
Plate Types Table131
About Wash Processing Patterns131
Maintenance135
Overview137
Recommended Maintenance Schedule139
Daily Maintenance141
Overnight/Multi-Day Maintenance141
Autoprime142
Autoprime for Overnight-Weekend Maintenance143
Turn on Autoprime for the Washer143
Removing Protein Residuals and Fungi Growth144
Autoclean the Washer145
Quick Clean (Keypad Only)146
Create an Autoclean Protocol147
Periodic Maintenance148
Clean the Bottles148
Clean the Plate Carrier149
Clean the Vacuum Filtration Carrier149
Clean the Exterior Surfaces and Mist Shield150
Clean the Buffer Bottle Filter150
Remove and Clean the Washer Manifold151
Maintaining the Vacuum Filtration System153
Clean the Vacuum Filtration Carrier153
Maintain the Verify™ Technology Components153
Clean the Verify™ Level Sensor153
Replacement Procedure for Verify Test Plate154
Volume Coefficients - Slope and Offset157
Decontamination158
Tools and Supplies158
Decontaminate Exterior Surfaces159
Decontaminate Tubing and Manifold160
Alternate Decontamination Procedure for Tubing and Manifold161
Decontaminate the Vacuum Filtration System162
Long Shutdown Procedure (Prepare for Storage or Shipment)163
Storing the Instrument164
Replace Components164
Remove/Change Wash Manifold165
Replace the Vacuum Pump Fuse165
Vacuum Filtration Carrier: Replace the Gasket166
Qualification167
Qualification Overview168
Qualification Schedule | 149169
System Self-Test, Verify Information169
Record Basecode170
Sensor Test: Fluid, Waste, Plate Detection170
Ultrasonic Cleaner Test171
Liquid Testing the 405™ Microplate Washer LS172
Which Tests to Perform172
Important Recommendations for All Liquid Tests173
Washer Liquid Tests174
Dispense Precision Test174
Evacuation Efficiency Test174
Predefined Test Protocols174
Washer Qualification Test Materials175
Dispense Precision Test177
Evacuation Efficiency Test178
Evacuation Diagnostic Test179
Buffer Switching Dispense Test180
Evacuation Efficiency Test182
Evacuation Diagnostic Test183
Vacuum Filtration Evacuation Efficiency Test185
Qualify the Verify™ Technology Sensor187
Troubleshooting199
Troubleshooting200
General Operation Troubleshooting202
Washer Problems203
Communication Errors208
Error Codes211
Dispense Precision Test212
System Error Codes212
405 LS-LHC Software Error Codes220

Biotek 405 LS - Page 100

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