62 | Chapter 3: Operation
Upload-Download Protocols (LHC Only)
The LHC lets you transfer protocols from your computer to your instrument and
back again.
Limitation: Protocols must contain only instrument-supported action steps to
qualify for download. That is, the protocol cannot contain any of the LHC provided
steps like Delay and Loop (buttons in the left column of the Add Step box in the
main view). And, a Protocol Name is required.
n The instrument's main menu must be displayed for the LHC to communicate
with it.
1. Select Tools>Transfer Protocols.
2. Make sure the desired protocols are displayed: check the Protocol Folder path
for This computer. Refresh the list of protocols onboard the Instrument by clicking
the Settings link.
3. Highlight one or more protocols in a display box. (Hold the Ctrl or Shift key to
simultaneously select multiple files.)
4. Optionally, at the top left corner of the screen, choose to Disable Editing of
transferred protocols to lock the protocols from editing or deleting when they
are onboard the instrument.
5. Click the applicable Upload or Download button.
The LHC will confirm the transfer or prompt you for more information. When the
transfer is complete, you can manipulate the files as you normally would in their
new location.
Wash Step Parameters Table
See Plate Types Table on page 111 for default Z-axis values or dispense heights.
Minimally, a wash step includes an aspirate step followed by a dispense step.
Select and define each option to customize the parameters for your assay.
Keypad
name
Option Description/Values range
Default
values
CYCLES Number of
wash cycles:
Each wash cycle first aspirates and then
dispenses fluid to and from the plate.
3
ASPIR Aspirate Vacuum Filtration: Select standard
aspiration (Top) or Vac for filtration.
405™ Microplate Washer LS
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