48 | Chapter 3: Operation
If Quick Wash is too limited to satisfy your assay requirements, use the LHC or the
keypad to define a wash protocol.All wash parameters can be defined during
protocol creation, including those designed for special assays.
Quick Prime (Keypad only)
Priming removes air bubbles from the tubing, ensuring optimal performance. Fluid
is dispensed to and aspirated from the priming trough. You can use Quick Prime
to comply with the daily maintenance recommendations.
Select QUICK>FULPRM or MANPRM to prime the washer.
FULLPRIME VOL:0300 A
BUFFER (300...999)
Quick Prime Menu
l Choose FULPRM to flush all the tubing, when changing fluids, for example. The valid
volume range is displayed, 300 to 999 mL in the above example.
l Choose MANPRM prior to running a protocol to correct for evaporation, i.e. to normalize
the dispense tips and remove any air from the manifold.
l VOL:0300 mL shows the dispense volume per well in milliliters.
To change the dispense volume, use the arrow keys to move the
cursor to the desired number position. The cursor appears to underline a number:
0010. When the correct position is selected, use the number pad to enter the desired
value. Or, press Options to increment the value, Shift+Options to decrease the value.
l Buffer is offered on washers with Buffer Switching to select the buffer valve.
The current selection is displayed in the top right corner of the LCD.
When the desired values are entered press Start.
See 405 Recommendations for Priming the Washer on page 39.
Create or Edit a Protocol (Keypad Only)
At the main menu:
1. Select DEFINE, and then, CREATE or EDIT.
CREATE EDIT
2.
Name the protocol and select the
Plate Type. Press Enter after
making selections to proceed. See
Select the protocol to edit: enter
its number or use the Options key
to scroll through the stored
405™ Microplate Washer LS
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