68 | Chapter 3: Operation
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In a Wash step, select the Show Wash Options link and fill the Pre-
dispense checkbox.
Washer Flow Rates
The default setting for flow rates is 7, in the middle of the range for standard
tubing rates 3-11, 3 is the slowest, 11 is the fastest. Slower rates are recommended
for viscous fluids.
Dual 96-Tube Manifold: When this manifold is installed, you can select rate 1 or 2
to use the low-flow tubing to dispense fluid slowly and gently to avoid damaging
well contents.
Rate µL/tube/second
96 tube 192 tube
Cell Wash 1 116* NA*
Cell Wash 2 134* NA*
3 204 102
4 244 122
5 273 136.5
6 306 153
Default 7 325 162.5
8 352 176
9 375 187.5
10 391 195.5
11 418 209
*Cell Wash flow rates direct fluid through special low-flow tubing, which is not
compatible with the single 96-tube and 192-tube wash manifolds.
Aspirate Travel Rates
Rate mm/sec
1 4.1 +1 Slowest
1 CW 4.1
2 5.0 +1
2 CW 5.0
3 7.3 +1 Default rate
3 CW 7.3
405™ Microplate Washer LS
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