44 | Chapter 3: Operation
QC (Quality Control) Protocols
Manifold-Specific
QC_96_DISP_TEST Dispense precision test protocol for 96-tube manifold.
QC_96_EVAC_TEST Evacuation efficiency test protocol for 96-tube manifold.
QC_192_DISP_TEST Dispense precision test protocol for 192-tube manifold.
QC_192_EVAC_TEST Evacuation efficiency test protocol for 192-tube manifold.
QC_96_VAC30_
TEST
Vacuum filtration evacuation efficiency test for 96-well filter plates
and also recommended for use in maintenance procedures.
QC_384_VAC10_
TEST
Vacuum filtration evacuation efficiency test for 384-well filter
plates and also recommended for use in maintenance procedures.
Predefined Sample Protocols
The "Sample" protocols are provided to facilitate learning. Some samples are model
specific.
Keypad Users: The protocol names onboard the washer do not include the W-
prefix and some protocols include the model name, e.g. 405HT or 405Select, when
appropriate.
LHC users: First open the folder that matches your washer model, e.g. 405 Select.
You may need to customize the protocols (as described on page 41) to match your
instrument's settings.
Cell Wash
W-CELLWASH_
96
Cell wash protocol designed to minimize cell layer disturbance in 96-
well plates. Aspirate height increased to 50 steps (6.35 mm above plate
carrier) resulting in increased residual, approximately 100 µL.
W-CELLWASH_
384
Cell wash protocol designed to minimize disturbance to cells in 384-well
plates. Aspirate height increased to 50 steps (6.35 mm above plate
carrier) will cause increased residual volume but help to preserve the
cell monolayer.
Microplate Manufacturers
W-Corning_
FLAT
Standard wash protocols modified to best position the manifold tubes
for dispensing and aspirating to Corning Costar flat-bottomed and
round-bottomed wells.
W-Corning_
ROUND
405™ Microplate Washer LS
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